Avoiding Etching of Silver When Using Silver Staining With OsO4 Fixation
نویسندگان
چکیده
منابع مشابه
Silver Staining of Alzheimer's Disease.
Paraffin sections were deparaffinized and hydrated to distilled water, then placed in 20% silver nitrate and kept in 60°C for 15 minutes, then rinsed in distilled water. Then sections were treated with formalin solution containing ammoniacal silver solution for about 15 min, followed by rinsing and placing tissue in sodium thiosulfate solution for 2 minutes and washing in tap water. The section...
متن کاملSilver staining of proteins in 2DE gels.
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of ...
متن کاملSilver staining of 2D electrophoresis gels.
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discusse...
متن کاملSweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry.
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing su...
متن کاملdevelopment of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles
a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy Today
سال: 1999
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500064762